Functional analysis of aromatic biosynthetic pathways in Pseudomonas putida KT2440.

ABSTRACT: Pseudomonas putida KT2440 is a non-pathogenic prototrophic bacterium with high potential for biotechnological applications. Despite all that is known about this strain, the biosynthesis of essential chemicals has not been fully analysed and auxotroph mutants are scarce. We carried out massive mini-Tn5 random mutagenesis and screened for auxotrophs that require aromatic amino acids. The biosynthesis of aromatic amino acids was analysed in detail including physical and transcriptional organization of genes, complementation assays and feeding experiments to establish pathway intermediates. There is a single pathway from chorismate leading to the biosynthesis of tryptophan, whereas the biosynthesis of phenylalanine and tyrosine is achieved through multiple convergent pathways. Genes for trypto

SUBMITTER: Molina-Henares MA 

PROVIDER: S-EPMC3815424 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature