TmRNA of Streptomyces collinus and Streptomyces griseus during the growth and in the presence of antibiotics.

ABSTRACT: Streptomycetes are soil microorganisms with the potential to produce a broad spectrum of secondary metabolities. The production of antibiotics is accompanied by a decrease in protein synthesis, which raises the question of how these bacteria survived the transition from the primary to the secondary metabolism. Translating ribosomes incapable to properly elongate or terminate polypeptide chain activate bacterial trans-translation system. Abundance and stability of the tmRNA during growth of Streptomyces collinus and Streptomyces griseus producing kirromycin and streptomycin, respectively, was analysed. The level of tmRNA is mostly proportional to the activity of the translational system. We demonstrate that the addition of sub-inhibitory concentrations of produced antibiotics to the culture

SUBMITTER: Paleckova P 

PROVIDER: S-EPMC3815426 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature