Mechanisms by which chronic ethanol feeding impairs the migratory capacity of cutaneous dendritic cells.

ABSTRACT:

Background

Chronic alcoholism is associated with increased incidence and severity of skin infection. Cutaneous dendritic cells (CDCs) play a pivotal role in skin immunity, and chronic ethanol (EtOH) feeding in mice has been shown to inhibit CDC migration to skin-draining lymph nodes (dLNs) following epicutaneous sensitization. Because CDC subsets differentially initiate T-cell responses, it is important to determine how EtOH feeding affects migration of each subset and identify mechanisms responsible for observed defects.

Methods

Mice received EtOH in the drinking water for ≥ 16 weeks. Baseline numbers of CDC subsets and their migration to the dLNs following fluorescein 5-isothiocyanate (FITC) sensitization were assessed by flow cytometry. Epidermal cell suspension and skin

SUBMITTER: Parlet CP 

PROVIDER: S-EPMC3815521 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature