A versatile and scalable strategy for glycoprofiling bifidobacterial consumption of human milk oligosaccharides.

ABSTRACT: Human milk contains approximately 200 complex oligosaccharides believed to stimulate the growth and establishment of a protective microbiota in the infant gut. The lack of scalable analytical techniques has hindered the measurement of bacterial metabolism of these and other complex prebiotic oligosaccharides. An in vitro, multi-strain, assay capable of measuring kinetics of bacterial growth and detailed oligosaccharide consumption analysis by FTICR-MS was developed and tested simultaneously on 12 bifidobacterial strains. For quantitative consumption, deuterated and reduced human milk oligosaccharide (HMO) standards were used. A custom software suite developed in house called Glycolyzer was used to process the large amounts of oligosaccharide mass spectra automatically with (13)C correction

SUBMITTER: Locascio RG 

PROVIDER: S-EPMC3815754 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature