Molecular and biochemical characterization of the tetralin degradation pathway in Rhodococcus sp. strain TFB.

ABSTRACT: The tetralin biodegradation pathway in Rhodococcus sp. strain TFB, a Gram-positive bacterium resistant to genetic manipulation, was characterized using a proteomic approach. Relative protein expression in cell free extracts from tetralin- and glucose-grown cells was compared using the 2D-DIGE technique. Identification of proteins specifically expressed in tetralin-grown cells was used to characterize a complete set of genes involved in tetralin degradation by reverse genetics. We propose a tetralin degradation pathway analogous to that described for Sphingomonas macrogolitabida strain TFA. TFB thn genes are organized into three operons; two contain all of the structural genes and are transcribed in the same direction, while the third operon, thnST, is transcribed in the opposite direction

SUBMITTER: Tomas-Gallardo L 

PROVIDER: S-EPMC3815846 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature