Depigmented allergoids reveal new epitopes with capacity to induce IgG blocking antibodies.

ABSTRACT:

Background

The synthesis of allergen-specific blocking IgGs that interact with IgE after allergen immunotherapy (SIT) has been related to clinical efficacy. The objectives were to investigate the epitope specificity of IgG-antibodies induced by depigmented-polymerized (Dpg-Pol) allergoids and unmodified allergen extracts, and examine IgE-blocking activity of induced IgG-antibodies.

Methods

Rabbits were immunized with native and Dpg-Pol extracts of birch pollen, and serum samples were obtained. Recognition of linear IgG-epitopes of Bet v 1 and Bet v 2 and the capacity of these IgG-antibodies to block binding of human-IgE was determined.

Results

Serum from rabbits immunized with native extracts recognised 11 linear epitopes from Bet v 1, while that from Dpg-Pol-immunize

SUBMITTER: Lopez-Matas MA 

PROVIDER: S-EPMC3816019 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature