Metabolite profiling identifies a branched chain amino acid signature in acute cardioembolic stroke.

ABSTRACT:

Background and purpose

There is limited information about changes in metabolism during acute ischemic stroke. The identification of changes in circulating plasma metabolites during cerebral infarction may provide insight into disease pathogenesis and identify novel biomarkers.

Methods

We performed filament occlusion of the middle cerebral artery of Wistar rats and collected plasma and cerebrospinal fluid 2 hours after the onset of ischemia. Plasma samples from control and patients with acute stroke were also analyzed. All samples were examined using liquid chromatography followed by tandem mass spectrometry. Positively charged metabolites, including amino acids, nucleotides, and neurotransmitters, were quantified using electrospray ionization followed by scheduled multiple r

SUBMITTER: Kimberly WT 

PROVIDER: S-EPMC3816089 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature