Ferumoxytol: a new, clinically applicable label for stem-cell tracking in arthritic joints with MRI.

ABSTRACT:

Aim

To develop a clinically applicable MRI technique for tracking stem cells in matrix-associated stem-cell implants, using the US FDA-approved iron supplement ferumoxytol.

Materials & methods

Ferumoxytol-labeling of adipose-derived stem cells (ADSCs) was optimized in vitro. A total of 11 rats with osteochondral defects of both femurs were implanted with ferumoxytol- or ferumoxides-labeled or unlabeled ADSCs, and underwent MRI up to 4 weeks post matrix-associated stem-cell implant. The signal-to-noise ratio of different matrix-associated stem-cell implant was compared with t-tests and correlated with histopathology.

Results

An incubation concentration of 500 µg iron/ml ferumoxytol and 10 µg/ml protamine sulfate led to significant cellular iron uptake, T2 signal effect

SUBMITTER: Khurana A 

PROVIDER: S-EPMC3816126 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature