The human beta-globin replication initiation region consists of two modular independent replicators.

ABSTRACT: Previous studies have shown that mammalian cells contain replicator sequences, which can determine where DNA replication initiates. However, the specific sequences that confer replicator activity were not identified. Here we report a detailed analysis of replicator sequences that dictate initiation of DNA replication from the human beta-globin locus. This analysis suggests that the beta-globin replication initiation region contains two adjacent, redundant replicators. Each replicator was capable of initiating DNA replication independently at ectopic sites. Within each of these two replicators, we identified short, discrete, nonredundant sequences, which cooperatively determine replicator activity. Experiments with somatic cell hybrids further demonstrated that the requirements for initiati

SUBMITTER: Wang L 

PROVIDER: S-EPMC381644 | biostudies-literature | 2004 Apr

REPOSITORIES: biostudies-literature