Integrative survival-based molecular profiling of human pancreatic cancer.

ABSTRACT:

Purpose

To carry out an integrative profile of human pancreatic ductal adenocarcinoma (PDAC) to identify prognosis-significant genes and their related pathways.

Experimental design

A concordant survival-based whole genome in silico array analysis of DNA copy number, and mRNA and miRNA expression in 25 early-stage PDAC was carried out. A novel composite score simultaneously integrated gene expression with regulatory mechanisms to identify the signature genes with the most levels of prognosis-significant evidence. The predominant signaling pathways were determined via a pathway-based approach. Independent patient cohorts (n = 148 and 42) were then used as in vitro validation of the array findings.

Results

The composite score identified 171 genes in which expressions wer

SUBMITTER: Donahue TR 

PROVIDER: S-EPMC3816537 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature