Anaerobic co-culture of mesenchymal stem cells and anaerobic pathogens - a new in vitro model system.

ABSTRACT:

Background

Human mesenchymal stem cells (hMSCs) are multipotent by nature and are originally isolated from bone marrow. In light of a future application of hMSCs in the oral cavity, a body compartment with varying oxygen partial pressures and an omnipresence of different bacterial species i.e. periodontitis pathogens, we performed this study to gain information about the behavior of hMSC in an anaerobic system and the response in interaction with oral bacterial pathogens.

Methodology/principal findings

We established a model system with oral pathogenic bacterial species and eukaryotic cells cultured in anaerobic conditions. The facultative anaerobe bacteria Fusobacterium nucleatum, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans were studied. Their effects

SUBMITTER: Kriebel K 

PROVIDER: S-EPMC3817215 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature