A single-stranded junction modulates nanosecond motional ordering of the substrate recognition duplex of a group I ribozyme.

ABSTRACT: Rigid spinning: Site-directed spin-labeling studies using a rigid nitroxide spin label (Ç) reveal that both length and sequence of a single-stranded junction (J1/2) modulate nanosecond motional ordering of the substrate-recognition duplex (P1) of the 120 kD group I ribozyme. The studies demonstrate an approach for experimental measurements of nanosecond dynamics in high-molecular-weight RNA complexes.

SUBMITTER: Nguyen P 

PROVIDER: S-EPMC3817310 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature