ATP-binding cassette B10 regulates early steps of heme synthesis.

ABSTRACT:

Rationale

Heme plays a critical role in gas exchange, mitochondrial energy production, and antioxidant defense in cardiovascular system. The mitochondrial transporter ATP-binding cassette (ABC) B10 has been suggested to export heme out of the mitochondria and is required for normal hemoglobinization of erythropoietic cells and protection against ischemia-reperfusion injury in the heart; however, its primary function has not been established.

Objective

The aim of this study was to identify the function of ABCB10 in heme synthesis in cardiac cells.

Methods and results

Knockdown of ABCB10 in cardiac myoblasts significantly reduced heme levels and the activities of heme-containing proteins, whereas supplementation with δ-aminolevulinic acid reversed these defects. Overexp

SUBMITTER: Bayeva M 

PROVIDER: S-EPMC3817742 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature