A novel multiplex PCR for detection of Pseudomonas aeruginosa: A major cause of wound infections.

ABSTRACT:

Background and objective

Wound infections are often difficult to treat due to various bacterial pathogens. Pseudomonas aeruginosa is one of the common invaders of open wounds. Precise diagnosis of this etiological agent in wound infections is of critical importance particularly in treatment of problematic cases. The existing diagnostic methods have certain limitations particularly related to specificity. Our objective was to to establish a comprehensive and reliable multiplex PCR to confirm diagnosis of P. aeruginosa.

Methods

A multiplex PCR test was developed for rapid and comprehensive identification of P. aeruginosa. Four highly specific genes were targeted simultaneously for detection of genus, species and exotoxin production (16S rDNA, gyrB, oprL and ETA) in P. aerugino

SUBMITTER: Salman M 

PROVIDER: S-EPMC3817763 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature