Expression, purification and crystallization of acetyl-CoA hydrolase from Neisseria meningitidis.

ABSTRACT: Neisseria meningitidis is the causative microorganism of many human diseases, including bacterial meningitis; together with Streptococcus pneumoniae, it accounts for approximately 80% of bacterial meningitis infections. The emergence of antibiotic-resistant strains of N. meningitidis has created a strong urgency for the development of new therapeutics, and the high-resolution structural elucidation of enzymes involved in cell metabolism represents a platform for drug development. Acetyl-CoA hydrolase is involved in multiple functions in the bacterial cell, including membrane synthesis, fatty-acid and lipid metabolism, gene regulation and signal transduction. Here, the first recombinant protein expression, purification and crystallization of a hexameric acetyl-CoA hydrolase from N. meningit

SUBMITTER: Khandokar YB 

PROVIDER: S-EPMC3818059 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature