Multidrug-resistant transport activity protects oocytes from chemotherapeutic agents and changes during oocyte maturation.

ABSTRACT:

Objective

To determine the multidrug-resistant transporter (MDR) activity in oocytes and their potential role in oocyte susceptibility to chemotherapy.

Design

Experimental laboratory study.

Setting

University and academic center for reproductive medicine.

Subject(s)

Women with eggs retrieved for intracytoplasmic sperm injection cycles and adult female FVBN and B6C3F1 mouse strains.

Intervention(s)

Inhibition of MDR activity in oocytes.

Main outcome measure(s)

Efflux activity of MDRs with the use of quantitative fluorescent dye efflux, and oocyte cell death when exposed to chemotherapy.

Result(s)

Oocytes effluxed fluorescent reporters, and this activity was significantly reduced in the presence of the MDR inhibitor PSC 833. Geminal vesicle o

SUBMITTER: Brayboy LM 

PROVIDER: S-EPMC3818338 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature