Stochastic profiling of transcriptional regulatory heterogeneities in tissues, tumors and cultured cells.

ABSTRACT: Single-cell variations in gene and protein expression are important during development and disease. Such cell-to-cell heterogeneities can be directly inspected one cell at a time, but global methods are usually not sensitive enough to work with the starting material of a single cell. Here we provide a detailed protocol for stochastic profiling, a method that infers single-cell regulatory heterogeneities by repeatedly sampling small collections of cells selected at random. Repeated stochastic sampling is performed by laser-capture microdissection or limiting dilution, followed by careful exponential cDNA amplification, hybridization to microarrays and statistical analysis. Stochastic profiling surveys the transcriptome for programs that are heterogeneously regulated among cellular subpopula

SUBMITTER: Wang L 

PROVIDER: S-EPMC3818581 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature