Engineering Streptomyces coelicolor for heterologous expression of secondary metabolite gene clusters.

ABSTRACT: We have constructed derivatives of Streptomyces coelicolor M145 as hosts for the heterologous expression of secondary metabolite gene clusters. To remove potentially competitive sinks of carbon and nitrogen, and to provide a host devoid of antibiotic activity, we deleted four endogenous secondary metabolite gene clusters from S. coelicolor M145--those for actinorhodin, prodiginine, CPK and CDA biosynthesis. We then introduced point mutations into rpoB and rpsL to pleiotropically increase the level of secondary metabolite production. Introduction of the native actinorhodin gene cluster and of gene clusters for the heterologous production of chloramphenicol and congocidine revealed dramatic increases in antibiotic production compared with the parental strain. In addition to lacking antibacte

SUBMITTER: Gomez-Escribano JP 

PROVIDER: S-EPMC3818861 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature