Imaging flow cytometry for morphologic and phenotypic characterization of rare circulating endothelial cells.

ABSTRACT: Endothelial cells in the peripheral circulation are rare events that require technically rigorous approaches for detection by flow cytometry. Visualization of these cells has been even more demanding, as this has historically required extensive enrichment and processing prior to attempting imaging. As a result, few, if any, examples exist on images of peripheral blood circulating endothelial cells (CECs) that include verification of the cell lineage both phenotypically and genomically. In this study, we have devised a method whereby CECs can be directly visualized after lysis of red blood cells and staining, without pre-enrichment or additional processing. Peripheral blood is stained with CD45, CD146, CD3, Hoechst, and DAPI to permit identification of CD146 positive, nonleukocyte, nucleate

SUBMITTER: Samsel L 

PROVIDER: S-EPMC3819459 | biostudies-literature | 2013 Nov-Dec

REPOSITORIES: biostudies-literature