Molecular characterization, biological forms and sporozoite rate of Anopheles stephensi in southern Iran.

ABSTRACT:

Objective

To identify the biological forms, sporozoite rate and molecular characterization of the Anopheles stephensi (An. stephensi) in Hormozgan and Sistan-Baluchistan provinces, the most important malarious areas in Iran.

Methods

Wild live An. stephensi samples were collected from different malarious areas in southern Iran. The biological forms were identified based on number of egg-ridges. Molecular characterization of biological forms was verified by analysis of the mitochondrial cytochrome oxidase subunit I and II (mtDNA-COI/COII). The Plasmodium infection was examined in the wild female specimens by species-specific nested-PCR method.

Results

Results showed that all three biological forms including mysorensis, intermediate and type are present in the study area

SUBMITTER: Chavshin AR 

PROVIDER: S-EPMC3819495 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature