Laser-targeted ablation of the zebrafish embryonic ventricle: a novel model of cardiac injury and repair.

ABSTRACT:

Background

While the adult zebrafish (Danio rerio) heart demonstrates a remarkable capacity for self-renewal following apical resection little is known about the response to injury in the embryonic heart.

Methods

Injury to the beating zebrafish embryo heart was induced by laser using a transgenic zebrafish expressing cardiomyocyte specific green fluorescent protein. Changes in ejection fraction (EF), heart rate (HR), and caudal vein blood flow (CVBF) assessed by video capture techniques were assessed at 2, 24 and 48 h post-laser. Change in total and mitotic ventricular cardiomyocyte number following laser injury was also assessed by counting respectively DAPI (VCt) and Phospho-histone H3 (VCm) positive nuclei in isolated hearts using confocal microscopy.

Results

Laser

SUBMITTER: Matrone G 

PROVIDER: S-EPMC3819623 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature