Differential protein expression throughout the life cycle of Trypanosoma congolense, a major parasite of cattle in Africa.

ABSTRACT: Trypanosoma congolense is an important pathogen of livestock in Africa. To study protein expression throughout the T. congolense life cycle, we used culture-derived parasites of each of the three main insect stages and bloodstream stage parasites isolated from infected mice, to perform differential protein expression analysis. Three complete biological replicates of all four life cycle stages were produced from T. congolense IL3000, a cloned parasite that is amenable to culture of major life cycle stages in vitro. Cellular proteins from each life cycle stage were trypsin digested and the resulting peptides were labeled with isobaric tags for relative and absolute quantification (iTRAQ). The peptides were then analyzed by tandem mass spectrometry (MS/MS). This method was used to identify an

SUBMITTER: Eyford BA 

PROVIDER: S-EPMC3820035 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature