Validation of SNP allele frequencies determined by pooled next-generation sequencing in natural populations of a non-model plant species.

ABSTRACT: Sequencing of pooled samples (Pool-Seq) using next-generation sequencing technologies has become increasingly popular, because it represents a rapid and cost-effective method to determine allele frequencies for single nucleotide polymorphisms (SNPs) in population pools. Validation of allele frequencies determined by Pool-Seq has been attempted using an individual genotyping approach, but these studies tend to use samples from existing model organism databases or DNA stores, and do not validate a realistic setup for sampling natural populations. Here we used pyrosequencing to validate allele frequencies determined by Pool-Seq in three natural populations of Arabidopsis halleri (Brassicaceae). The allele frequency estimates of the pooled population samples (consisting of 20 individual plant

SUBMITTER: Rellstab C 

PROVIDER: S-EPMC3820589 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature