Neuron-specific feeding RNAi in C. elegans and its use in a screen for essential genes required for GABA neuron function.

ABSTRACT: Forward genetic screens are important tools for exploring the genetic requirements for neuronal function. However, conventional forward screens often have difficulty identifying genes whose relevant functions are masked by pleiotropy. In particular, if loss of gene function results in sterility, lethality, or other severe pleiotropy, neuronal-specific functions cannot be readily analyzed. Here we describe a method in C. elegans for generating cell-specific knockdown in neurons using feeding RNAi and its application in a screen for the role of essential genes in GABAergic neurons. We combine manipulations that increase the sensitivity of select neurons to RNAi with manipulations that block RNAi in other cells. We produce animal strains in which feeding RNAi results in restricted gene knockd

SUBMITTER: Firnhaber C 

PROVIDER: S-EPMC3820814 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature