Molecular responses of mouse macrophages to copper and copper oxide nanoparticles inferred from proteomic analyses.

ABSTRACT: The molecular responses of macrophages to copper-based nanoparticles have been investigated via a combination of proteomic and biochemical approaches, using the RAW264.7 cell line as a model. Both metallic copper and copper oxide nanoparticles have been tested, with copper ion and zirconium oxide nanoparticles used as controls. Proteomic analysis highlighted changes in proteins implicated in oxidative stress responses (superoxide dismutases and peroxiredoxins), glutathione biosynthesis, the actomyosin cytoskeleton, and mitochondrial proteins (especially oxidative phosphorylation complex subunits). Validation studies employing functional analyses showed that the increases in glutathione biosynthesis and in mitochondrial complexes observed in the proteomic screen were critical to cell surviv

SUBMITTER: Triboulet S 

PROVIDER: S-EPMC3820927 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature