Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition.

ABSTRACT: Microtubules dramatically change their dynamics and organization at the entry into mitosis. Although this change is mediated by microtubule-associated proteins (MAPs), how MAPs themselves are regulated is not well understood. Here we used an integrated multi-level approach to establish the framework and biological significance of MAP regulation critical for the interphase/mitosis transition. Firstly, we applied quantitative proteomics to determine global cell cycle changes in the profiles of MAPs in human and Drosophila cells. This uncovered a wide range of cell cycle regulations of MAPs previously unidentified. Secondly, systematic studies of human kinesins highlighted an overlooked aspect of kinesins: most mitotic kinesins suppress their affinity to microtubules or reduce their protein l

SUBMITTER: Syred HM 

PROVIDER: S-EPMC3820929 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature