Exploring the N-glycosylation pathway in Chlamydomonas reinhardtii unravels novel complex structures.

ABSTRACT: Chlamydomonas reinhardtii is a green unicellular eukaryotic model organism for studying relevant biological and biotechnological questions. The availability of genomic resources and the growing interest in C. reinhardtii as an emerging cell factory for the industrial production of biopharmaceuticals require an in-depth analysis of protein N-glycosylation in this organism. Accordingly, we used a comprehensive approach including genomic, glycomic, and glycoproteomic techniques to unravel the N-glycosylation pathway of C. reinhardtii. Using mass-spectrometry-based approaches, we found that both endogenous soluble and membrane-bound proteins carry predominantly oligomannosides ranging from Man-2 to Man-5. In addition, minor complex N-linked glycans were identified as being composed of partiall

SUBMITTER: Mathieu-Rivet E 

PROVIDER: S-EPMC3820931 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature