Host-centric proteomics of stool: a novel strategy focused on intestinal responses to the gut microbiota.

ABSTRACT: The diverse community of microbes that inhabits the human bowel is vitally important to human health. Host-expressed proteins are essential for maintaining this mutualistic relationship and serve as reporters on the status of host-microbiota interaction. Therefore, unbiased and sensitive methods focused on host proteome characterization are needed. Herein we describe a novel method for applying shotgun proteomics to the analysis of feces, focusing on the secreted host proteome. We have conducted the most complete analysis of the extracellular mouse gut proteome to date by employing a gnotobiotic mouse model. Using mice colonized with defined microbial communities of increasing complexity or a complete human microbiota ('humanized'), we show that the complexity of the host stool proteome mi

SUBMITTER: Lichtman JS 

PROVIDER: S-EPMC3820941 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature