Increased frequency of intestinal CD4+ T cells reactive with mycobacteria in patients with Crohn's disease.

ABSTRACT:

Objective

The aim of this study was to assess the frequency of mycobacteria and Escherichia coli reactive T cells in intestinal biopsies from patients with Crohn's disease (CD) and ulcerative colitis (UC).

Materials and methods

The biopsies were obtained by colonoscopy from adult patients with active CD (n = 5) and active UC (n = 4). The number of CD4+ T cell clones expanded and screened from each patient varied from 383 to 3972 giving a total of 16639 individual clones. The T cell clones were tested for responses to Mycobacterium avium subspecies paratuberculosis (MAP) and E. coli. The cytokine profile of 42 individual T cell clones from four CD patients was assessed.

Results

The frequency of mycobacteria reactive T cell clones in CD patients ranged from 0.17 to 1.63

SUBMITTER: Olsen I 

PROVIDER: S-EPMC3821379 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature