Transcriptional regulation of a chitinase gene by 20-hydroxyecdysone and starvation in the oriental fruit fly, Bactrocera dorsalis.

ABSTRACT: Insect chitinases are hydrolytic enzymes that are required for the degradation of glycosidic bonds of chitin. In this study, we identified and characterized a full-length cDNA of the chitinase gene (BdCht2) in the oriental fruit fly, Bactrocera dorsalis. The cDNA contains an open reading frame (ORF) of 1449 bp that encodes 483 amino acid residues and 126- and 296-bp non-coding regions at the 5'- and 3'-ends, respectively. The BdCht2 genome has four exons and three introns. The predicted molecular mass of the deduced BdCht2 is approximately 54.3 kDa, with an isoelectric point of 5.97. The 977 bp 5' flanking region was identified and the transcription factor binding sites were predicted. Bioinformatic analyses showed that the deduced amino acid sequence of BdCht2 had 34%-66% identity to that

SUBMITTER: Yang WJ 

PROVIDER: S-EPMC3821602 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature