Single cell force spectroscopy of T cells recognizing a myelin-derived peptide on antigen presenting cells.

ABSTRACT: T-cell recognition of peptide-MHC complexes on APCs requires cell-cell interactions. The molecular events leading to T-cell activation have been extensively investigated, but the underlying physical binding forces between T-cells and APCs are largely unknown. We used single cell force spectroscopy for quantitation of interaction forces between T-cells and APCs presenting a tolerogenic peptide derived from myelin basic protein. When T-cells were brought into contact with peptide-loaded APCs, interaction forces increased with time from about 0.5nN after 10s interaction to about 15nN after 30min. In the absence of antigen, or when ICAM-1-negative APC was used, no increase in binding forces was observed. The temporal development of interaction forces correlated with the kinetics of immune syna

SUBMITTER: Hoffmann S 

PROVIDER: S-EPMC3821867 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature