Proteomic profiling reveals upregulated protein expression of hsp70 in keloids.

ABSTRACT:

Background

The biochemical characteristics of keloid-derived fibroblasts differ from those of adjacent normal fibroblasts, and these differences are thought to be the cause of abnormal fibrosis. Therefore, we investigated the characteristic proteins that are differentially expressed in keloid-derived fibroblasts using proteomics tools.

Objective

We attempted to investigate the novel proteins that play important roles in the pathophysiology of keloids.

Methods

Proteomics analysis was performed to identify differentially expressed proteins in keloid-derived fibroblasts. Keloid-derived fibroblasts and adjacent normal fibroblasts were analyzed with 2-DAGE. We validated these proteins with immunoblot analysis, real-time RT-PCR, and immunohistochemistry.

Results

Sixt

SUBMITTER: Lee JH 

PROVIDER: S-EPMC3821890 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature