Analysis of microdissected neurons by 18O mass spectrometry reveals altered protein expression in Alzheimer's disease.

ABSTRACT: It is evident that the symptoms of Alzheimer's disease (AD) are derived from severe neuronal damage, and especially pyramidal neurons in the hippocampus are affected pathologically. Here, we analysed the proteome of hippocampal neurons, isolated from post-mortem brains by laser capture microdissection. By using (18)O labelling and mass spectrometry, the relative expression levels of 150 proteins in AD and controls were estimated. Many of the identified proteins are involved in transcription and nucleotide binding, glycolysis, heat-shock response, microtubule stabilization, axonal transport or inflammation. The proteins showing the most altered expression in AD were selected for immunohistochemical analysis. These analyses confirmed the altered expression levels, and showed in many AD cases

SUBMITTER: Hashimoto M 

PROVIDER: S-EPMC3822682 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature