Proteome-wide detection and quantitative analysis of irreversible cysteine oxidation using long column UPLC-pSRM.

ABSTRACT: Reactive oxygen species (ROS) play an important role in normal biological functions and pathological processes. ROS is one of the driving forces for oxidizing proteins, especially on cysteine thiols. The labile, transient, and dynamic nature of oxidative modifications poses enormous technical challenges for both accurate modification site determination and quantitation of cysteine thiols. The present study describes a mass spectrometry-based approach that allows effective discovery and quantification of irreversible cysteine modifications. The utilization of a long reverse phase column provides high-resolution chromatography to separate different forms of modified cysteine thiols from protein complexes or cell lysates. This Fourier transform mass spectrometry (FT-MS) approach enabled detec

SUBMITTER: Lee CF 

PROVIDER: S-EPMC3823053 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature