DNA modification under mild conditions by Suzuki-Miyaura cross-coupling for the generation of functional probes.

ABSTRACT: Quick and clean: A method for Pd-catalyzed Suzuki-Miyaura cross-coupling to iododeoxyuridine (IdU) in DNA is described. Key to the reactivity is the choice of the ligand and the buffer. A covalent [Pd]-DNA intermediate was isolated and characterized. Photocrosslinking probes were generated to trap proteins that bind to epigenetic DNA modifications.

SUBMITTER: Lercher L 

PROVIDER: S-EPMC3823066 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature