Change in hepatocyte growth factor concentration promote mesenchymal stem cell-mediated osteogenic regeneration.

ABSTRACT: Mesenchymal stem cells (MSCs) play a crucial role in tissue repair by secretion of tissue nutrient factors such as hepatocyte growth factor (HGF). However, studies examining the effects of HGF on the proliferation and differentiation of MSCs used different concentrations of HGF and reported conflicting conclusions. This study aimed to determine the mechanisms by which different concentrations of HGF regulate MSC proliferation and osteogenic differentiation, and validate the mechanism in an animal model of early stage avascular necrosis of femoral head (ANFH). Our results demonstrate that a low concentration of HGF (20 ng/ml) preferentially promotes MSC osteogenic differentiation through increased c-Met expression and phosphorylation, Akt pathway activation, and increased expression of p27,

SUBMITTER: Wen Q 

PROVIDER: S-EPMC3823079 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature