A new approach to simultaneously quantify both TCR α- and β-chain diversity after adoptive immunotherapy.

ABSTRACT:

Purpose

T-cell receptor (TCR) variable Vα and Vβ gene diversity is a surrogate biomarker for the therapeutic potential of adoptive immunotherapy and cellular immunity. Therefore, creating a straightforward, rapid, sensitive, and reliable method to view the global changes of both TCRVα and Vβ transcripts in heterogeneous populations of T cells is appealing.

Experimental design

We designed a "direct TCR expression assay" (DTEA) using a panel of customized bar-coded probes that simultaneously detects and quantifies 45 Vα and 46 Vβ transcripts in a nonenzymatic digital multiplexed assay from a small number of cells (10(4) cells) or as little as 100 ng of total RNA.

Results

We evaluated DTEA on total RNA samples of tumor-infiltrating lymphocytes and peripheral blood obtain

SUBMITTER: Zhang M 

PROVIDER: S-EPMC3823368 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature