Inhibition of IKKβ in enterocytes exacerbates sepsis-induced intestinal injury and worsens mortality.

ABSTRACT:

Objectives

Nuclear factor-κB is a critical regulator of cell-survival genes and the host inflammatory response. The purpose of this study was to investigate the role of enterocyte-specific NF-kB in sepsis through selective ablation of IkB kinase.

Design

Prospective, randomized controlled study.

Setting

Animal laboratories in university medical centers.

Subjects and interventions

Mice lacking functional NF-kB in their intestinal epithelium (Vil-Cre/Ikkβ) and wild-type mice were subjected to sham laparotomy or cecal ligation and puncture. Animals were killed at 24 hours or followed 7 days for survival.

Measurements and main results

Septic wild-type mice had decreased villus length compared with sham mice, whereas villus atrophy was further exacerbated in s

SUBMITTER: Dominguez JA 

PROVIDER: S-EPMC3823541 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature