CD44 targeting magnetic glyconanoparticles for atherosclerotic plaque imaging.

ABSTRACT:

Purpose

The cell surface adhesion molecule CD44 plays important roles in the initiation and development of atherosclerotic plaques. We aim to develop nanoparticles that can selectively target CD44 for the non-invasive detection of atherosclerotic plaques by magnetic resonance imaging.

Methods

Magnetic glyconanoparticles with hyaluronan immobilized on the surface have been prepared. The binding of these nanoparticles with CD44 was evaluated in vitro by enzyme linked immunosorbent assay, flow cytometry and confocal microscopy. In vivo magnetic resonance imaging of plaques was performed on an atherosclerotic rabbit model.

Results

The magnetic glyconanoparticles can selectively bind CD44. In T2* weighted magnetic resonance images acquired in vivo, significant contrast cha

SUBMITTER: El-Dakdouki MH 

PROVIDER: S-EPMC3823634 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature