SELDI-TOF analysis of glioblastoma cyst fluid is an approach for assessing cellular protein expression.

ABSTRACT:

Objectives

In about 10% of glioblastoma patients, preoperative MRI discloses the presence of tumor cysts. Whereas the impact of cystic appearance on prognosis has been discussed extensively, only little is known about the tumor cyst fluid. In this study, we tested the feasibility of the surface enhanced laser desorption ionization time of flight (SELDI-TOF) technique to detect cyst fluid proteins.

Methods

Cyst fluid was collected from 21 glioblastoma patients for SELDI-TOF analysis and compared to control cerebrospinal fluids from 15 patients with spinal stenosis. Resulting protein peaks with significant differences between groups were further described, using the molecular weight in an internet search of protein databases and publications. Two potential cyst fluid proteins,

SUBMITTER: Hoelscher M 

PROVIDER: S-EPMC3823932 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature