Is transplantation of cryopreserved ovarian tissue from patients with advanced-stage breast cancer safe? A pilot study.

ABSTRACT:

Purpose

To assess the safety of reimplantation of cryopreserved ovarian tissue from advanced-stage breast cancer patients.

Methods

Cryopreserved ovarian cortical fragments were obtained from 13 advanced-stage breast cancer patients aged 17-35 years. After thawing, part of the ovarian cortical tissue was grafted to severe combined immunodeficient mice for 6 months. The presence of malignant mammary cells in ovarian tissue was evaluated after thawing as well as after grafting by 1) histology and immunohistochemistry (epithelial membrane antigen, Her2/neu and gross cystic disease fluid protein 15 identification), and 2) detection of the MGB2 gene by qPCR.

Results

No malignant cells were evidenced by histology and immunohistochemistry. None of the mice died during the 6-m

SUBMITTER: Luyckx V 

PROVIDER: S-EPMC3824859 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature