Glucose principally regulates insulin secretion in mouse islets by controlling the numbers of granule fusion events per cell.

ABSTRACT:

Aims/hypothesis

In dispersed single beta cells the response of each cell to glucose is heterogeneous. In contrast, within an islet, cell-to-cell communication leads to glucose inducing a more homogeneous response. For example, increases in NAD(P)H and calcium are relatively uniform across the cells of the islet. These data suggest that secretion of insulin from single beta cells within an islet should also be relatively homogeneous. The aim of this study was to test this hypothesis by determining the glucose dependence of single-cell insulin responses within an islet.

Methods

Two-photon microscopy was used to detect the glucose-induced fusion of single insulin granules within beta cells in intact mouse islets.

Results

First, we validated our assay and showed that the

SUBMITTER: Low JT 

PROVIDER: S-EPMC3825531 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature