A novel electrochemical method for efficient reduction of disulfide bonds in peptides and proteins prior to MS detection.

ABSTRACT: A novel electrochemical (EC) method for fast and efficient reduction of the disulfide bonds in proteins and peptides is presented. The method does not use any chemical agents and is purely instrumental. To demonstrate the performance of the EC reactor cell online with electrospray mass spectrometry, insulin and somatostatin were used as model compounds. Efficient reduction is achieved in continuous infusion mode using an EC reactor cell with a titanium-based working electrode. Under optimized conditions, the presented method shows almost complete reduction of insulin and somatostatin. The method does not require any special sample preparation, and the EC reactor cell makes it suitable for automation. Online EC reduction followed by collision-induced dissociation fragmentation of somatostat

SUBMITTER: Kraj A 

PROVIDER: S-EPMC3826059 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature