Functional genomics of novel secondary metabolites from diverse cyanobacteria using untargeted metabolomics.

ABSTRACT: Mass spectrometry-based metabolomics has become a powerful tool for the detection of metabolites in complex biological systems and for the identification of novel metabolites. We previously identified a number of unexpected metabolites in the cyanobacterium Synechococcus sp. PCC 7002, such as histidine betaine, its derivatives and several unusual oligosaccharides. To test for the presence of these compounds and to assess the diversity of small polar metabolites in other cyanobacteria, we profiled cell extracts of nine strains representing much of the morphological and evolutionary diversification of this phylum. Spectral features in raw metabolite profiles obtained by normal phase liquid chromatography coupled to mass spectrometry (MS) were manually curated so that chemical formulae of met

SUBMITTER: Baran R 

PROVIDER: S-EPMC3826126 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature