Increased telomerase activity and vitamin D supplementation in overweight African Americans.

ABSTRACT:

Objective

We aimed to investigate whether vitamin D supplementation modulates peripheral blood mononuclear cell (PBMC) telomerase activity in overweight African Americans.

Design

A double blind, randomized and placebo-controlled clinical trial (#NCT01141192) was recently conducted.

Subjects and methods

African-American adults were randomly assigned to either the placebo, or the vitamin D group (60,000 IU per month (equivalent to ~2000 IU per day) oral vitamin D3 supplementation). Fresh PBMCs were collected from 37 subjects (18 in the placebo group and 19 in the vitamin D group), both at baseline and 16 weeks. PBMC telomerase activity was measured by the telomeric repeat amplification protocol.

Results

Serum 25 hydroxyvitamin D levels increased from 40.7±15.7 at

SUBMITTER: Zhu H 

PROVIDER: S-EPMC3826782 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature