The C2 domains of otoferlin, dysferlin, and myoferlin alter the packing of lipid bilayers.

ABSTRACT: Ferlins are large multi-C2 domain membrane proteins involved in membrane fusion and fission events. In this study, we investigate the effects of binding of the C2 domains of otoferlin, dysferlin, and myoferlin on the structure of lipid bilayers. Fluorescence measurements indicate that multi-C2 domain constructs of myoferlin, dysferlin, and otoferlin change the lipid packing of both small unilamellar vesicles and giant plasma membrane vesicles. The activities of these proteins were enhanced in the presence of calcium and required negatively charged lipids like phosphatidylserine or phosphatidylglycerol for activity. Experiments with individual domains uncovered functional differences between the C2A domain of otoferlin and those of dysferlin and myoferlin, and truncation studies suggest tha

SUBMITTER: Marty NJ 

PROVIDER: S-EPMC3826957 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature