C-KIT signaling is targeted by pathogenic Yersinia to suppress the host immune response.

ABSTRACT:

Background

The pathogenic Yersinia species exhibit a primarily extracellular lifestyle through manipulation of host signaling pathways that regulate pro-inflammatory gene expression and cytokine release. To identify host genes that are targeted by Yersinia during the infection process, we performed an RNA interference (RNAi) screen based on recovery of host NF-κB-mediated gene activation in response to TNF-α stimulation upon Y. enterocolitica infection.

Results

We screened shRNAs against 782 genes in the human kinome and 26 heat shock genes, and identified 19 genes that exhibited ≥ 40% relative increase in NF-κB reporter gene activity. The identified genes function in multiple cellular processes including MAP and ERK signaling pathways, ion channel activity, and regulation o

SUBMITTER: Micheva-Viteva SN 

PROVIDER: S-EPMC3827001 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature