Identification and characterization of angiogenesis targets through proteomic profiling of endothelial cells in human cancer tissues.

ABSTRACT: Genomic and proteomic analysis of normal and cancer tissues has yielded abundant molecular information for potential biomarker and therapeutic targets. Considering potential advantages in accessibility to pharmacological intervention, identification of targets resident on the vascular endothelium within tumors is particularly attractive. By employing mass spectrometry (MS) as a tool to identify proteins that are over-expressed in tumor-associated endothelium relative to normal cells, we aimed to discover targets that could be utilized in tumor angiogenesis cancer therapy. We developed proteomic methods that allowed us to focus our studies on the discovery of cell surface/secreted proteins, as they represent key antibody therapeutic and biomarker opportunities. First, we isolated endothelia

SUBMITTER: Mesri M 

PROVIDER: S-EPMC3827283 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature