A conserved pre-block interaction motif regulates potassium channel activation and N-type inactivation.

ABSTRACT: N-type inactivation occurs when the N-terminus of a potassium channel binds into the open pore of the channel. This study examined the relationship between activation and steady state inactivation for mutations affecting the N-type inactivation properties of the Aplysia potassium channel AKv1 expressed in Xenopus oocytes. The results show that the traditional single-step model for N-type inactivation fails to properly account for the observed relationship between steady state channel activation and inactivation curves. We find that the midpoint of the steady state inactivation curve depends in part on a secondary interaction between the channel core and a region of the N-terminus just proximal to the pore blocking peptide that we call the Inactivation Proximal (IP) region. The IP interacti

SUBMITTER: Pfaffinger PJ 

PROVIDER: S-EPMC3827413 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature