In silico method for modelling metabolism and gene product expression at genome scale.

ABSTRACT: Transcription and translation use raw materials and energy generated metabolically to create the macromolecular machinery responsible for all cellular functions, including metabolism. A biochemically accurate model of molecular biology and metabolism will facilitate comprehensive and quantitative computations of an organism's molecular constitution as a function of genetic and environmental parameters. Here we formulate a model of metabolism and macromolecular expression. Prototyping it using the simple microorganism Thermotoga maritima, we show our model accurately simulates variations in cellular composition and gene expression. Moreover, through in silico comparative transcriptomics, the model allows the discovery of new regulons and improving the genome and transcription unit annotatio

SUBMITTER: Lerman JA 

PROVIDER: S-EPMC3827721 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature